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Image Search Results
Journal: eLife
Article Title: The Ku subunit of telomerase binds Sir4 to recruit telomerase to lengthen telomeres in S. cerevisiae
doi: 10.7554/eLife.07750
Figure Lengend Snippet: 35 S-methionine-labeled Sir4 was synthesized in vitro in a rabbit reticulocyte lysate transcription/translation system (RRL) to which purified Ku heterodimer, bearing a 2myc epitope on the C-terminus of Yku80, was added. After Sir4 synthesis, the RRL was subjected to anti-myc immunoprecipitation. The input, unbound, and bound fractions were run on an SDS polyacrylamide gel, which was imaged by autoradiography. DOI: http://dx.doi.org/10.7554/eLife.07750.010
Article Snippet: First, ∼16 pmol of purified, myc-tagged yeast Ku heterodimer ( ; ) was added to the
Techniques: Labeling, Synthesized, In Vitro, Purification, Immunoprecipitation, Autoradiography